Stability of Newcastle Disease Virus Strain V4-UPM Coated on Cassava Granules and Exposed to High Temperatures.

  • GON Echeonwu
  • CU Iroegbu
  • BC Echeonwu
  • A Ngene
  • AO Olabode
  • AEJ Okwori
  • J Ndako
  • AS Junaid
  • OI Okeke
Keywords: Cassava granules, V4-UPM, virus infectivity, high temperatures.


Protection of village chickens against Newcastle disease (ND) is considered feasible through food-delivered vaccines. Vaccine virus strain V4-UPM coated on cassava granules with or without additive (2% gelatin) was tested for stability at room temperature (RT) for 8 weeks and 40oC for 12 hours at weekly and two hourly intervals respectively. Stability was assessed by estimation of residual infectivity and infective virus excretion using standard methods. Results showed that at RT, the titre (EID50/gm) of the food vaccine with additive did not drop below the minimum effective
immunogenic titre (MEIT) of 106.0 until after 4 weeks, whereas the one without additive dropped below MEIT value at the 3rd week of exposure. Following exposure at 40oC, the one with additive lost MEIT value after the 10th hour, while the infectivity of food vaccine without additive dropped below MEIT value after the 6th hour of exposure. Only four chickens produced HI antibody at first, whereas all 10 sero-converted on booster dose of vaccine, all 5 vaccinated and challenged birds resisted challenge, while all the unvaccinated control birds died. The vaccine virus was efficiently excreted by all birds that ate the food vaccine. It is hereby concluded that cassava granules sustained virus infectivity fairly enough for effective village chicken vaccination in any part of Nigeria.

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eISSN: 1116-1043