Role of cow urine on the onset of leptazole-induced convulsion in Wistar rats

Abstract

The study investigated the possible preventive role of cow urine on the onset of pentylenetetrazole (leptazole) induced convulsion in Wistar rats. Forty rats (each weighing 100-120g) in four groups were used. Two groups were orally administered with varying doses of cow urine and, after 60 minutes, convulsive dose (0.2ml/kg) of leptazole, administered intraperitoneally. A reference group, not given cow urine, was given same dose of leptazole only, and another group, which was given neither cow urine nor leptazole, served as control. Results showed that cow urine did not prevent convulsion as there was no significant difference (P<0.05) in the time of onset of convulsion in the group given cow urine and the reference group that did not receive cow urine. Results also showed that there was no significant difference in the onset time of convulsion between the two groups that were given different doses of cow urine. Four out of the ten rats in the reference group that received only leptazole died within a period 60 minutes after the administration of the drug, while the rest recovered, though they were depressed. Eight out of the ten rats in the group that received single dose of cow urine died, while all ten rats in the group that received double dose of cow urine died within the same period of 60 minutes. This suggests that some constituent chemicals in cow urine may have toxic effects in rats, made worse with the administration of leptazole.

Keywords: Cow urine; Leptazole; Convulsion