Synthesis of sugars catalysed by microgel conjugated rabbit muscle aldolase in aqueous and aqueous-organic solvents II
Abstract
Kinetic runs of microgel-aldolase mediated reactions between dihydroxyacetone phosphate cyclohexylamine salt and D-glyceraldehydes were made in CH2CN, THF, DMF, DMSO and MeOH and aqueous-organic solution of these solvents at physiological temperature 37º. The same runs were effected between DHAP cyclohexylamine salt and non-natural aldehyde acceptors under similar conditions. Reaction was fastest in 1:3 DMSO/ H2O, faster than in aqueous medium the environment in which this enzyme normally operates. Preparation of a non-natural sugar using this system is related.
Keywords: Microgel-aldolase, Kinetic physiological temperature, Natural acceptors, Non-natural acceptors.
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