Somatic Embryogenesis in Juniperus Procera using Juniperus Communis as a Model
Abstract
The study of somatic embryogenesis in Juniperus communis has been conducted as a preliminary study for the further development of somatic embryogenesis, micropropagation and long-term conservation/cryopreservation in Juniperus procera, which is economically and ecologically important and endangered forest species in Ethiopia. The aim for this particular research was initially an adaptation of optimum half strength lithium chloride-sodium propionate (LP) medium protocol for growth and proliferation of embryogenic culture. But as other conifer species it was difficult to stop proliferation of somatic embryo cell division and bring them to maturation stage. However using optimal concentration of ABA (32 mg/l), it was possible to get maturation response in most of the cultures. Additional study on the effect of seed extraction to the growing embryogenic culture showed no effect on mature somatic embryos. Rather they were showing faster growth which increases embryogenic mass of each culture. It was also observed that green premature seeds of Juniperus are suitable for the initiation and proliferation of somatic embryo. Key words: Cryopreservation, Juniperus communis, Juniperus procera, Somatic embryogenesis.The Momona Ethiopian Journal of Science accepts the manuscripts for consideration with the understanding that the manuscript has not been published and is not under consideration for publication elsewhere. Only original articles will be considered for publication if they have been published previously as abstracts, but not if they have been published previously as extended abstract (>1000 words). This applies to both electronic and print versions of the journal. The authors should assign copyright ownership to the Editorial Office of MEJS in the event that the manuscript is accepted for publication in the Momona Ethiopian Journal of Science. All accepted manuscripts must be accompanied by a copyright statement signed by all authors. A copy of the copyright form will be supplied along with the final reviewed version of the manuscript that is sent for final proof- reading. Authors may make multiple copies of the form if necessary and send to the Editorial Office with author’s signature(s) even individually.
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