Effect of Platlate Riched Fibrin and mononuclear cells on regeneration of osteochondral defect in rabbits

Abstract

Objective: To assess the effectiveness of Platlate riched fibrin (PRF) and bone marrow derived mononuclea r cells (BMNCs) in regeneration of osteochondral defects in rabbits.

Design: A randomized-controlled experimental study.

Animals: Forty-eight adult New Zealand rabbits were allocated randomly into four groups (n=12).

Procedures: An osteoch ondora l defect of a 4 mm diameter and 5 mm depth was made in the trochlear groove of the left stifle joints.  The defect was left for spontaneous healing in group A, filled with Platlate riched fibrin (PRF) in group B, filled with BMN Cs in group C  and combina tio n of PRF and BMNCs in group D. Healing of the defects wa s assessed grossly and microscopically at 3, 6 and 12 weeks  postoperative.

Results: Grossly, the degree of defect repair, integra tion to border zone and appearance of defect area were significantly  higher in group D than other groups (P≤0.0 5 ). Microscopica lly, surface architecture, tissue morpholog y, cell distribution and  safranin O staining of the matrix were significa ntly higher in group D than other groups (P≤0.0 5 ).

Conclusion and clinical relevance: The results of the present study indicated that, combination of PRF and BMNCs encourages quicker  and better healing of osteochondral defects.