PROTEIN PROFILE STUDY OF Sarotherodon melanotheron FROM SOUTH-WEST NIGERIAN WATER BODIES

  • I. Megbowon Department of Biotechnology, Nigerian Institute for Oceanography and Marine Research, Victoria Island, Lagos, Nigeria

Abstract

Sarotherodon melanotheron is a Cichlid specie with good nutritional, economic and aquaculture potentials. The
genetic difference among S. melanitheron populations in South-West Nigeria was investigated using 12%
Sodium Dodecyl Sulphate- polyacrylamide Gel Electrophoresis (SDS-PAGE). Three populations from Pepe,
Ugbonla, and Badagry were considered for the study. Thirty fish samples were analyzed; ten per location for the
protein profiling. The banding pattern from sarcoplasmic protein indicated variation among the populations.
Molecular weight of the protein bands varied from 10-250kDa. Ninety eight (98%) of genetic variation that was
found among populations reflects a high inter-population differentiation. The protein profile produced three
clusters, indicating divergence and also suggests high level of genetic variation among the studied populations of
S. melanotheron. The existence of genetic diversity among these populations has made them suitable for use in
future breeding programs for the development of improved S. melanotheron species.

Published
2020-08-30

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eISSN: 0300-368X