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COMPARISON OF SUCROSE AND GLUCOSE AS CARBON SOURCES FOR INVITRO GROWTH OF Moringa oleifera Lam. EMBRYOS


U. C. Okafor

Abstract

The embryo of Moringa oleifera Lam. was cultured in vitro using sucrose and glucose as carbon sources at two
different concentrations to check the effect of the different carbon sources and concentrations on the growth of the
cultured explants. The concentrations used were 2% and 4% each of sucrose and glucose respectively, while 0%
served as control. It was recorded that the explant in 2% and 4% sucrose attained 50% sprouting on the 4th day, and
produced the highest root length and highest number of roots. Whereas, explants in 2% and 4% glucose attained
50% sprouting on the 6th day after inoculation, and produced the highest leaf length, shoot length, number of
leaves and highest value of fresh weight in grams (g). Therefore, 2% and 4% sucrose supported root growth and
elongation, while 2% and 4% glucose supported leaf growth and shoot growth.


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