Effect of vitamin C in egg yolk extender on sperm motility of chilled stored bull semen

Abstract

An experiment was conducted to evaluate the effect of vitamin C in chicken and quail egg yolk extender on sperm motility in chilled stored bull semen. Semen was collected from the bull with the aid of artificial vagina and six types of extenders were prepared using chicken and quail egg yolk with each extender divided into three aliquots having 0, 3 and 6 mg/mL Vitamin C levels. Bottles containing the diluted semen using the chicken and quail egg yolk extenders were stored in a refrigerator at 5°C over a period of three days and monitored or evaluated at 0, 24, 48 and 72 hours. The result showed that there was a general decline in motility of semen as storage time increases, although samples with 6mg/mL Vitamin C levels had better results. It can be concluded that adding Vitamin C in chicken and quail egg yolk extender maintained motility of chilled semen even at 72 hours of preservation at 5⁰C.