Reduction of Exudates (Browning) in Sugarcane Micro Propagation

  • MN Ishaq
  • Bernard O Ehirim

Abstract

Ex plant preparation in the course of sugarcane micro propagation is known to release exudates which lead to the death of sugarcane in vitro. An industrial sugarcane variety (NCS008) was used for the study and was observed to die in less than 14 days in vitro. In a study to reduce the death of this variety, ascorbic acid and citric acid was added as constituent of the media using MS and stock at different concentrations. The browning was reduced drastically at the addition of 0.1g/litre ascorbic acid and 0.15g/litre citric acid.

Keywords: reduction, exudates browning, micro propagation, sugarcane

Nig J. Biotech. Vol. 23 (2011) 40 - 44

Author Biographies

MN Ishaq
Biotechnology unit, National Cereals Research Institute Badeggi, P.M.B 8 Bida Niger State Nigeria
Bernard O Ehirim
Biotechnology unit, National Cereals Research Institute Badeggi, P.M.B 8 Bida Niger State Nigeria
Published
2014-06-19
Section
Articles

Journal Identifiers


eISSN:
print ISSN: 01891731