Assessment of the Biocompatibility of Mineral Trioxide Aggregate, Bioaggregate, and Biodentine in the Subcutaneous Tissue of Rats
Objective: The objective of this study was to evaluate the tissue inflammation caused by three endodontic repair materials.
Materials and Methods: The materials included micro mega‑mineral trioxide aggregate (MM‑MTA), bioaggregate (BA), and biodentine (BD), which were implanted into the subcutaneous tissue of rats. The tissue samples for histological examination were prepared. The infiltration of lymphocytes and macrophages into the tissue was examined to assess the inflammatory response.
Results: Lymphocyte infiltration: A significant increase was detected in the MM‑MTA and BA groups on the 7th and 14th days as compared with the control (7th day P = 0.0001, 14th day P = 0.0176). There was no difference between the groups on the 45th day (P = 0.1730). Lymphocyte infiltration had decreased over time in all groups. Macrophage infiltration: There was a significant increase by the 7th day in the test groups as compared to the control group (P = 0.007). However, there was no difference between the experimental groups on the 14th (P = 0.2708) and 45th (P = 0.1291) days.
Conclusion: While MM‑MTA and BA showed a similar biocompatibility, BD was more biocompatible than MM‑MTA and BA in the 1st week of the experiment. However, there was no difference between the materials at the end of the 45th day. MM‑MTA, BA, and BD can be considered suitable endodontic repair materials.
Keywords: Bioaggregate, Biocompatible Materials, Biodentine, Endodontics