<i>TP53</i> Polymorphisms in Smokers’ and Nonsmokers’ Pericoronal Follicles of Asymptomatic Impacted Third Molars

Abstract

Background and Purpose: The investigators designed and implemented a prospective cohort study composed of smoking and nonsmoking patients with asymptomatic fully impacted mandibular third molars. The objective of the paper was to evaluate 21 single‑nucleotide polymorphisms (SNP) on the TP53 gene in smokers’ (S) and nonsmokers’ (NS) pericoronal follicles of asymptomatic impacted third molars. Materials and Methods: Matrix‑assisted desorption/ionization time of the flight mass spectrometry was used for SNP analysis of 21 regions in the TP53 gene. Descriptive statistics and Chi‑square tests were computed with a P value of 0.05. Results: Ten of the 21 SNPs related to oral pathologies according to NCBI dbSNP, were detected; in these, the genotypic frequencies showed no differences between the S and NS groups (P > 0.05). The results showed a high ratio of SNPs without correlation between smoking and TP53 gene status. Conclusion: Further studies should examine the entire TP53 gene to elucidate how smoking affects it in larger study populations.

Keywords: Dental follicle, matrix‑assisted desorption/ionization time of flight mass spectrometry, single‑nucleotide polymorphism, smoking, TP53