Isolation and Characterization of Bioactive Constituents of <i>Boscia salicifolia</i>. Olive (Capparaceae)

Abstract

Extraction of the crude extract of B. salicifolia was carried out using methanol as solvent with the aid of Soxhlet apparatus. The extract was concentrated in-vacuo at 40^oC using rotary evaporator. The crude methanol extract was subjected to thin layer chromatography and vacuum liquid chromatography (VLC). The isolated compound BSME was characterized by IR and GC-MS. The IR spectral studies indicated the presence of hydroxyl, carbonyl, alkane and aromatic functional groups. GC-MS revealed nine prominent peaks with retention times of 5.133, 11.62, 14.692, 15.725, 16.475, 17.433, 18.183, 18.317 and 20.733 minutes indicating the presence of 1,2,3-propanetriol, 3,5-diterbutylphenol, 2-(2-hydroxy-2-phenylethoxy)phenol, methyl-12-methyltetradecanoate, n-Hexadecanioc acid, 13-octadecanoate, 9- Octadecanioc acid, n- Octadecanoic acid and 9- octadecenal respectively.