Cu(II) complex of lincomycin: Synthesis, spectral characterization and competitive DNA binding

Abstract

Cu(II) complex [CuLin-H(H₂O)₂](NO₃)₂ (1) of the antibiotic lincomycin has been synthesized and characterized by elemental (C,H,N) analysis, UV-Vis, FTIR, ¹H NMR and ESI mass spectra. Spectra studies revealed that monomeric 5-coordinate Cu(II) and Fe(III) complexes of lincomycin were formed. Competitive DNA binding fluorescence measurement of complex 1 and CT DNA-bound ethidium bromide revealed that the complex is capable of binding DNA, albeit weakly, to displace ethidium bromide from CT DNA to quench the fluorescence of ethidium bromide bound to CT DNA.

Keywords: Copper(II),Lincomycin, Complex, Antibiotic, DNA-binding