Antihaemolytic, Antihaemorrhagic and Antifibrinolytic Effects of Fractions of Buchholzia coriacea Seeds on Naja nigricollis Crude Venom

  • J.I Achika
  • R.G Ayo
  • A.O Oyewale
  • J.D Habila
  • P.Y Ofemile
Keywords: Antihaemolytic, antihaemorrhagic, fibrinolytic, venom, Bulchhozia coriacea


Bulchhozia coriacea (Capparaceae) seeds are used in managing snake bite in Western Nigeria were investigated against Naja nigricollis (Spitting cobra) venom-induced hemolytic, hemorrhagic and fibrinolytic effects. This study was aimed at determining the antihaemolytic, antihaemorrhagic as well as antifibrinolytic effects of B. coriacea on N. nigricollis crude venom. Microwave-assisted extraction with hexane, chloroform, ethyl acetate, and methanol was carried out. Naja nigricollis venom-induced erythrocyte lysis (100 %) was significantly reduced to 18% by the chloroform fraction at 0.625 mg/mL. At the concentration of 0.625 mg/mL, the hexane, chloroform, ethyl acetate and methanol fractions administered in combination with the venom reduced percentage hemorrhagic activity to 23%, 17%, 49%, and 87%, respectively. In conclusion, Bulchhozia coriacea seed fractions exhibited significant antihaemolytic, antihaemorrhagic and antifibrinolytic activities against N. nigricollis crude venom and may beneficial as a pre-treatment the while victim is transferred to a healthcare facility to receive the definite treatment to ensure speedy recovery.

Key words: Antihaemolytic, antihaemorrhagic, fibrinolytic, venom, Bulchhozia coriacea


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eISSN: 0331-3026