Honey samples were obtained from the different agro-ecological zones of Nigeria including: the tropical rainforest, mangrove swamp, plateau grassland, guinea savannah and sudan savannah. The antimicrobial activities of these differently sourced honeys were assessed against six bacteria organisms (Staphylococcus aureaus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Klebsiella aerogenes and Proteus mirabilis). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were studied using standard methods. Results obtained showed that geographical locations had effects on the antibacterial activities of the different honeys at different concentrations (6.25-100%). Honey originating from Vom, Plateau State showed the highest antimicrobial activity. All the honeys showed varied bacteriostatic and bactericidal activities. None of the honeys produced any effect on Klebsiella aerogenes and Proteus mirabilis. Further work is encouraged.