Molecular diagnostic of cytomegalovirus, Epstein Barr virus and Herpes virus 6 infections among blood donors by multiplex real-time PCR in Ouagadougou, Burkina Faso

  • Lassina Traore
  • Issoufou Tao
  • Cyrille Bisseye
  • Birama Diarra
  • Tegwindé Rebeca Compaore
  • Yacouba Nebie
  • Maleki Asshi
  • Alice Ouedraogo
  • Theodora Zohoncon
  • Florencia Djigma
  • Djénéba Ouermi
  • Nicolas Barro
  • Mahamoudou Sanou
  • Rasmata Traore Ouedraogo
  • Jacques Simpore

Abstract

Introduction: in most developing countries, Cytomegalovirus (CMV), Epstein Barr virus (EBV) and Herpes virus 6 (HHV-6) are not diagnosed in blood donors. The aim of this study is to determine the prevalence of these viruses in blood donors from the city of Ouagadougou, Burkina Faso.  Methods: the study included 198 blood donors of the Regional Blood Transfusion Centre of Ouagadougou. Multiplex real time PCR was used to diagnose the three viruses. Statistical analysis was performed with the software EpiInfo version 6 and SPSS version 17. P values ≤ 0.05 were considered significant. Results: of 198 samples tested, 18 (9.1%) were positive to at least one of the three viruses. In fact, 10 (5.1%) were positive for EBV, 10 (5.1%) positive for CMV and 12 (6.1%) positive for HHV-6. Viral infections were higher in women than in men, EBV (8,6% versus 4.3%), CMV (8.6% versus 3.7%) and HHV-6 (11.4% versus 4.9%). EBV / CMV / HHV-6 co-infection was found in 3.5% (7/198) of blood donors. Conclusion: the prevalence recorded in this study is low compared to those found in previous studies from the sub-region among blood donors. The molecular diagnostic test used in our study could explain the differences with previous studies.

The Pan African Medical Journal. 2016;24

Author Biographies

Lassina Traore
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Issoufou Tao
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Cyrille Bisseye
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso; Laboratory of Molecular and Cellular Biology,University of Sciences and Techniques of Masuku, P 943 Franceville,Gabon
Birama Diarra
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Tegwindé Rebeca Compaore
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Yacouba Nebie
Molecular Biology, Epidemiology and Monitoring of Communicable of Bacteria and Viruses Through Food,UFR / SVT,University of Ouagadougou, Burkina Faso
Maleki Asshi
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Alice Ouedraogo
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Theodora Zohoncon
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Florencia Djigma
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Djénéba Ouermi
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Nicolas Barro
Molecular Biology, Epidemiology and Monitoring of Communicable of Bacteria and Viruses Through Food,UFR / SVT,University of Ouagadougou, Burkina Faso
Mahamoudou Sanou
Molecular Biology, Epidemiology and Monitoring of Communicable of Bacteria and Viruses Through Food,UFR / SVT,University of Ouagadougou, Burkina Faso
Rasmata Traore Ouedraogo
Molecular Biology, Epidemiology and Monitoring of Communicable of Bacteria and Viruses Through Food,UFR / SVT,University of Ouagadougou, Burkina Faso
Jacques Simpore
Biomolecular Research Center Pietro Annigoni Labiogene,UFR / SVT, University of Ouagadougou BP 364 Ouagadougou, Burkina Faso
Published
2016-10-02
Section
Articles

Journal Identifiers


eISSN: 1937-8688