Adherence of staphylococcus aureus to catheter tubing inhibition by quaternary ammonium compounds

  • Jean-Marie Liesse Iyamba
  • Daniel Tassa Okombe
  • Francis Nsimba Zakanda
  • Trésor Kimbeni Malongo
  • Joseph Welo Unya
  • Cyprien Mbundu Lukukula
  • Ntondo za Balega Takaisi Kikuni

Abstract

Introduction: S. aureus is a Gram positive bacterium which is responsible for a wide range of infections. This pathogen has also the ability to adhere to biotic or abiotic surface such as central venous catheter (CVC) and to produce a biofilm. The aim of this study was to evaluate the effect of hexadecyltrimethyl ammonium bromide (HTAB) and Hexadecylbetainate chloride (HBC) on Staphylococcus aureus adherence to the catheter tubing and on bacteria growth. Methods: broth microdilution method was used to determine the Minimal Inhibitory Concentration (MIC). The detection of slime production was done by Congo Red Agar method, and the adherence of bacteria to the catheter tubing was evaluated by the enumeration of bacteria on plate counts. Results: the results of this study showed that the MICs of HTAB were ranged from 0.125 to 0.5 µg/mL, and those of HBC fluctuated between 2 to 8 µg/mL. HTAB and HBC inhibited bacteria adhesion on the surface of the catheter tubing. Conclusion: this study showed that HTAB and HBC can prevent the adherence of S. aureus strains to the surface of catheter tubing, suggesting that they could be used to prevent the risk of catheter related bloodstream infections.

The Pan African Medical Journal 2016;25

Author Biographies

Jean-Marie Liesse Iyamba
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Daniel Tassa Okombe
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Francis Nsimba Zakanda
Laboratory of Biochemistry, Faculty of Medicine, University of Kinshasa, Democratic Republic of Congo
Trésor Kimbeni Malongo
Laboratory of Instrumental and Bioelectrochemical analysis, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Joseph Welo Unya
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Cyprien Mbundu Lukukula
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Ntondo za Balega Takaisi Kikuni
Laboratory of Experimental and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Published
2016-10-05
Section
Articles

Journal Identifiers


eISSN: 1937-8688