Angiotensin-converting enzyme insertion/deletion gene polymorphisms and the risk of glioma in an Algerian population

  • Hana Ikram Benenemissi Department of Animal Biology, Faculty of Life and Natural Sciences, Molecular and Cellular Biology Laboratory, University of Constantine 1, Constantine, Algeria
  • Karima Sifi Department of Biochemistry, Ben Badis University Hospital, Biology and Genetics Research Laboratory, University of Constantine 3, Constantine, Algeria
  • Lakhder Khalil Sahli Department of Neurosurgery, Regional Military Hospital of Constantine (HMRUC), Constantine, Algeria
  • Ouarda Semmam Department of Animal Biology, Faculty of Life and Natural Sciences, Molecular and Cellular Biology Laboratory, University of Constantine 1, Constantine, Algeria
  • Noureddine Abadi Department of Biochemistry, Ben Badis University Hospital, Biology and Genetics Research Laboratory, University of Constantine 3, Constantine, Algeria
  • Dalila Satta Department of Animal Biology, Faculty of Life and Natural Sciences, Molecular and Cellular Biology Laboratory, University of Constantine 1, Constantine, Algeria
Keywords: Angiotensin-converting enzyme; gene polymorphism; insertion/deletion; Glioma

Abstract

Introduction: just recently, it has been established that the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism is linked to the pathogenesis and to the evolution of human cancers. Therefore, the present study was concerned with the investigation of an eventual association between glioma and I/D polymorphism of the ACE gene.

Methods: the expression of ACE gene was detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis in 36 Algerian patients with glioma and 195 healthy controls.

Results: in glioma cases, allelic frequencies and genotypes distribution of the ACE I/D polymorphism were different from controls cases. ACE DD genotype were highly presented in glioma cases (63.9%) than controls (33.8%) and conferred 3.64-fold risk for predisposition in glioma cases (vs ID genotype, p<0.001). Recessive model (ACE II + ID genotypes vs DD) was associated with a 72% reduced risk of glioma (OR = 0.28, 95% CI: 0.13-0.60, p <0.001). Per copy D allele frequency was found higher in glioma cases (79.2%) than in controls (63.3 %), OR = 2.20, 95% CI: 1.20 - 4.03, p = 0.009.

Conclusion: the obtained data showed that the presence of the D allele might be a risk factor for the development of glioma. Further studies considering different ethnic groups with large samples are required to confirm this finding.

Published
2019-04-23
Section
Articles

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eISSN: 1937-8688