Selected genes of Human herpesvirus-8 associated Kaposi’s sarcoma among patients with Human Immunodeficiency Virus-1 and Acquired Immunodeficiency Disease Syndrome

  • Rodgers Norman Demba School of Health Sciences, Kisii University, Kisii, Kenya; Institute of Tropical and Infectious Diseases, University of Nairobi, Nairobi, Kenya
  • Nathan Shaviya Masinde Muliro University of Science and Technology, Kakamega, Kenya
  • Sylviah Mweyeli Aradi Kenya Department of Internal Medicine, University of Nairobi, Nairobi, Kenya
  • Walter Mwanda Institute of Tropical and Infectious Diseases, University of Nairobi, Nairobi, Kenya
Keywords: KSHV; histology; nested PCR

Abstract

Introduction: Kaposi's sarcoma (KS) is a kind of cancer that causes flat or raised lesions containing Human herpes virus 8 (HHV8). The KS lesions are common among immunosuppressed HIV patients. Highly Active Antiretroviral (HHART) treats and prevents the development of KS. The objective of this study was to determine the presence of K1 and K15 (predominant alleles) genes in Kaposi's sarcoma-associated herpes virus (KSHV) among immunosuppressed patients due to HIV -1.

Methods: this was a cross-sectional descriptive study where consecutive sampling technique was adopted to pick archived tissue blocks from the Thematic Unit of Anatomic Pathology, Department of Human Pathology, College of Health Sciences, University of Nairobi and Department of Laboratory Medicine, Histology Section, Kenyatta National Hospital

Results: upon staining 81 tissue blocks with H & E, 84% (68/81) were diagnosed as KS and 16% (13/81) as KS-like. The K1 and K15 (P) genes were both detected at 88.9% (72/81) in the tissue blocks, with 95.8% (69/72) detection from KS and4.2% (3/72) from the KS-like.

Conclusion: the K1 and K15 (P) genes of KSHV were present among the immunosuppressed patients with Human Immunodeficiency Virus (HIV)-1. It is important to carry out K1 and K15 (P) genes detection on tissues that are diagnosed as KS or KS-like by histology technique

Published
2019-04-30
Section
Articles

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eISSN: 1937-8688