Supported Liquid Membrane Extraction of Anabolic Androgenic Compounds in Biological Matrices and Detection by LC-ESI-MS
Abstract
A sample work-up and enrichment technique involving the use of supported liquid membrane (SLM) and detection by high performance liquid chromatography coupled to a mass spectrometer operating under positive ion electrospray mode (LC-PI-ESI-MS) has been developed for the determination of six anabolic androgenic compounds in different biomatrices; mainly urine, kidney, liver and milk. Anabolic androgenic compounds analysed included 17α-trenbolone, 17β-trenbolone, 19-nortestosterone, testosterone, 4-androstene-3,17-dione and testosterone benzoate. Several factors affecting the extraction efficiency during SLM enrichment, such as donor pH were studied. The detection limits (DL) were 0.08 μg L–1 for 4-androstene-3,17-dione, 0.7 ng L–1 19-testosterone, 1.1 ng L–1 for testosterone, 0.1 ng L–1 for 17β-trenbolone, 1.6 ng L–1 for 17α-trenbolone and 0.03 μg L–1 for testosterone benzoate. Modification at C17 in the structures of 4-androstene-3,17-dione and testosterone benzoate affected their recoveries with SLM and explained their observed high detection limits.
Keywords: Anabolic androgenic compounds, supported liquid membrane, liquid chromatography, electrospray ionization, massspectrometry
Copyright for articles published in this journal is retained by the journal.
SAJChem applies the Creative Commons Attribution (CC BY) license to manuscripts we publish. This license was developed to facilitate open access – namely, free immediate access to, and unrestricted reuse of, original works of all types. Under this license, authors agree to make articles legally available for reuse, without permission or fees, for virtually any purpose. Anyone may copy, distribute or reuse these articles, as long as the author and original source are properly cited.
