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Measurement of pepsin in porcine gastric juice


J Fourie
RS Arnot
J Carter
R Hickman
J Terblanche

Abstract

The method for measurement of pepsin in human gastric juice as devised by Anson and modified by Berstad has been used for testing pepsin secretion from Heidenhain pouches in pigs. Twenty samples may be analysed at one time with accuracy. Variations in ambient temperature and humidity, and contamination of samples with bile, were found to interfere with the accuracy of the method. There was no change in activity after immediate freezing at -20°C or after storage at -20°C for up to 3 months. After repeated thawing and refreezing on 4 consecutive days, there was a fall in activity on the fourth day only.

S. Afr. Med. J., 48, 1873 (1974).

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eISSN: 2078-5135
print ISSN: 0256-9574