Vinnige identifikasiemetodes van giste tydens fungemie

  • L Badenhorst
  • P.L. Botha
  • M.N. Janse van Rensburg


A wet preparation method, modified serum test and a modified disc diffusion method were evaluated in order to identify yeasts more rapidly during fungaemia. A total of 2932 blood cultures were processed, of which 54 (1,8%) yielded yeasts. By using these methods, Candida albicans was identified within 3 hours, after yeast growth in the blood culture was confirmed by Gram stain. The wet preparation examinations were accurate in 60% of cases and the serum test in 95% of cases. After growth was detected, yeast species other than C. albicans were identified within 24 hours using the modified disc diffusion method. C. albicans (50%), C. tropicalis (22%) and C. parapsilosis (22%) were the species most frequently isolated. Fungaemia occurred ,most often after antimicrobial therapy and abdominal conditions.


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eISSN: 0256-95749
print ISSN: 2078-5135