Anti-bacterial activity of Plumbago zeylanica L. roots on some pneumonia causing pathogens

  • Hirut Lemma Ethiopian Health and Nutrition Research Institute
  • Asfaw Debella Ethiopian Health and Nutrition Research Institute
  • Getachew Addis Ethiopian Health and Nutrition Research Institute
  • Olaf Kunert Institute of Pharmaceutical Chemistry, Karl-Franzens University of Graz, Graz, Austria
  • Aberra Geyid Ethiopian Health and Nutrition Research Institute
  • Ferehiwot Teka Ethiopian Health and Nutrition Research Institute
  • Kidist Yersaw Ethiopian Health and Nutrition Research Institute

Abstract

The anti bacterial activity of polar and non-polar extracts prepared from the roots of Plumbago zeylanica L. (Plumbaginaceae), a plant widely used in Ethiopian traditional medicine for various ailments were investigated using hole plate diffusion method against some pneumonia causing pathogens. The aqueous extract did not exhibit any activity while petroleum ether extract was found to have strong anti-bacterial effects as compared to the ethanol extract which showed a significant activity. Activity guided chromatographic purification of the petroleum ether extract led to the isolation of three compounds, of which the compound identified as 5-hydroxy-2-methyl-1, 4-naphthoquinone, plumbagin, found to be the active component on the tested microorganisms. Minimum inhibitory concentration value of this particular compound showed comparative activity resembling the commonly used broad spectrum antibiotic, tetracycline. The strong antibacterial effect of the petroleum ether extract is discussed to show that it was attributable to this compound rather than the other two that were found to have trace of activities.

Key words/phrases: Anti-bacterial activity, Minimum inhibitory concentration, Plumbago zeylanica, pneumonia

SINET: Ethiopian Journal of Science Vol.25(2) 2002: 285-294

Author Biographies

Hirut Lemma, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Asfaw Debella, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Getachew Addis, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Aberra Geyid, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Ferehiwot Teka, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Kidist Yersaw, Ethiopian Health and Nutrition Research Institute
PO Box 1242, Addis Ababa, Ethiopia
Section
Articles

Journal Identifiers


eISSN: 2520-7997
print ISSN: 0379-2897