Test of endothelial monolayer integrity in the presence or absence of endotoxin and anti-tnf-a monoclonal antibodies

  • Andualem Mossie Department of Physiology, Jimma Health Science Institute PO Box 378, Jimma, Ethiopia
  • Stefan Eriksson Department of Physiology and Pharmacology, Karolinska Institute Post address 171 77, Stockholm, Sweden
  • Hassen Taha Sherief Department of Physiology, Faculty of Medicine PO Box 9086, Addis Ababa, Ethiopia
  • Legesse Zerihun Department of Physiology, Faculty of Medicine PO Box 9086, Addis Ababa, Ethiopia
Keywords: Anti-TNF-a monoclonal antibodies, electrical resistance, endothelial monolayer, endotoxin, permeability

Abstract



Monolayer cultures of human umbilical vein endothelial cells were established and tested for the effects of endotoxin treatment and anti-TNF-a monoclonal antibodies following endotoxin treatment on albumin permeability and electrical conductance. Application of 40 -g(ml)-1 of endotoxin on human umbilical cord endothelial cell culture monolayer increased transendothelial albumin permeability significantly (P<0.001) and decreased transendothelial electrical resistances significantly (P<0.001). When 10 -g(ml)-1 of anti-TNF-a monoclonal antibodies was added after endotoxin, the effects of the latter on albumin permeability and electrical resistance were significantly decreased (P<0.001). This suggested that the monolayer sheet can be disturbed by endotoxin-induced TNF-a from endothelial cells. TNF-a can disintegrate the endothelial cell monolayer during septicemia. This can be abrogated using anti-TNF-a monoclonal antibodies.

SINET: Ethiopian Journal of Science Vol. 24, No. 1 (June 2001), pp. 105-112

Key words/phrases: Anti-TNF-a monoclonal antibodies, electrical resistance, endothelial monolayer, endotoxin, permeability
Published
2004-06-09
Section
Articles

Journal Identifiers


eISSN: 2520-7997
print ISSN: 0379-2897