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Tanzania Journal of Health Research

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Relationship between alpha+-thalassaemia and glutathione-S-transferases polymorphisms in children with severe malaria in Tanzania

Fredy Saguti, Sakurani T. Balthazary, Alphaxard Manjurano, Robert A. Max, Filemon Tenu, Filbert Francis, Seif A. Shekalaghe, Reginald A. Kavishe

Abstract


Alpha+-thalassaemia is well known for conferring partial protection to severe malaria. On the other, Glutathione –S-transferase (GST) polymorphism has recently been associated to severe malaria in children. A retrospective cross sectional study was carried out to determine the relationship between genotypic polymorphisms of alpha+-thalassaemia and glutathione-S-transferase in children with severe malaria. A total of 148 DNA samples from children aged between 3 and 15 years with mild and severe malaria were retrieved and determined by polymerase chain reaction. Children with Glutathione-S-transferase-pi1 (GSTP1)-polymorphism were observed to have three fold risk (OR = 2.9; 95% CI =1.3- 6.1; P = 0.006) of developing severe malaria compared to mild malaria in Mnyuzi in Korogwe District, north- eastern, Tanzania. In the presence of Glutathione-S-transferase-pi1 polymorphism, children were found to have 3% decreased protective effect of alpha+-thalassaemia polymorphisms (homozygotes and heterozygotes) against severe malaria although this was not statistically significant [OR =0.81 (95% CI = 0.5-1.5; P = 0.5) to OR =0.78(95% CI = 0.4-1.5; P = 0.44)]. We conclude that Glutathione-S-transferase-pi1 polymorphism increases risk of developing severe malaria due to Plasmodium falciparum in children. The observed inverse relationship between GSTP1 polymorphisms and alpha+-thalassaemia to children with severe malaria need further investigation.




http://dx.doi.org/10.4314/thrb.v15i2.2
AJOL African Journals Online