TY - JOUR AU - Masalu, Rose J. AU - Hosea, Ken M. AU - Malendeja, Sylives PY - 2011/12/24 Y2 - 2024/03/28 TI - Free radical scavenging activity of some fungi indigenous to Tanzania JF - Tanzania Journal of Health Research JA - Tanzania J Hlth Res VL - 14 IS - 1 SE - Articles DO - 10.4314/thrb.v14i1.6 UR - https://www.ajol.info/index.php/thrb/article/view/57253 SP - AB - <p>The objective of this study was to evaluate free radical scavenging capacity of crude extracts from forest basidiomycetous fungi, domestic zygomycetous fungi and marine ascomycetous fungi. Lethal concentration values that kill 50% of the brine shrimps (LC<sub>50</sub>) were determined from 19 fungal extracts using brine shrimp test (BST). The LC<sub>50 </sub>values of fungal extract ranged between 0.28&ndash;40&micro;g/ml. The basidiomycetous (<em>Lactarius volemoides</em>) was the most toxic fungi with LC<sub>50</sub> of 0.28&micro;g/ml while ascomycete <em>Pichia guilliermondii</em> showed the least toxicity with LC<sub>50</sub> of 40&micro;g/ml. The concentrations of eleven fungal extracts were further evaluated on their ability to scavenge free radical using 1-diphenyl-2-picrylhydrazyl (&alpha;,&alpha;-diphenyl-&beta;-picrylhydrazyl) (DPPH) as a dye reagent for spectrophotometric assay at 517nm. The extract concentrations that decreased the initial DPPH radical by 50% (EC<sub>50</sub>) were determined. The EC<sub>50 </sub>values ranged from 19&ndash;60.4&micro;g/ml ascorbic acid equivalents. Extracts from an edible but undomesticated basidiomycetous fungus isolated from Miombo forest<em> </em>and identified as <em>Termitomyces microcarpus</em> showed the highest scavenging effect with EC<sub>50 </sub>at 19&micro;g/ml while that from ascomycete <em>Candida tropicalis</em> showed the least EC<sub>50 </sub>at 60.4&micro;g/ml. These results draw attention to wild undomesticated Miombo fungi as potential source of nutritional supplements worth further investigation.</p> ER -