Comparative Analysis of the Dark Ground Buffy Coat Technique (DG) and Enzyme-Linked Immunosorbent Assay (ELISA) in the Diagnosis of Trypanosomosis in Cattle

Abstract

The prevalence of typanosome infection in 65 cattle reared under expensive system of management was determined using the dark ground buffy coat (DG) technique and the enzyme-linkedimmunisorbent assay (ELISA). The DG technique showed that there were 18 positive cases (27.69%) of total number of animals, made up of 6 of Trypanosoma congolense, 7 of T. Vivax and 3 of T. Brucei. Other cases were those of mixed infections mainly one case of which all of 3 Trypansomes species were present, and another in which T. vivax, 12 of T. brucei and 2 cases of T. brucei were 30, (46.15%) positive cases made up of 6 of T. congolense, 7 of T. vivax, 12 of T. brucei and 2 cases of T. brucie mixed with t. Conglolese. The ELISA technique did not detect any incidence of mixed infections in which 3 species of trypanosomes were present.

Key words: ELISA, Buffy coat, Trypanosomosis, cattle