Synthesis and evaluation of antioxidant and antimicrobial activities of Schiff base tin ( II ) complexes

Purpose: To synthesize and evaluate Schiff base Tin (II) complexes for antioxidant and antimicrobial activities Methods: The complexes of Tin (II) chloride with various Schiff base derivative of 2-Hydroxy-1naphthaldehyde (HN) were synthesized and characterized by various physiochemical techniques, including elemental analysis, ultraviolet–visible (UV-Vis) spectrophotometry, Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (H–NMR), carbon-13 nuclear magnetic resonance (C-NMR), electron ionization mass spectrometry (EIMS) and conductance studies. Agarwell diffusion and agar-well dilution techniques were used for antimicrobial assessment. Evaluation of the antioxidant activity of the ligands and complexes was accomplished by DPPH radical scavenging assay. Results: The results indicate coordination of deprotonated phenolic oxygen and nitrogen of azomethine with Tin (II) chloride led to the formation of stable complexes. The Tin (II) complexes showed good antimicrobial and antioxidant activities. Compounds (HNPA and [Sn(HNPA)Cl2(H2O)2) were noteworthy (p < 0.05) in this regard with antibacterial activity against Staphylococcus aureus, while [Sn(HNCA)Cl2(H2O)2 and [Sn(HNPT)Cl2(H2O)2 were active against Klebsiella pneumoniae.. Conclusion: All synthesized Schiff bases and their Tin (II) complexes showed high antimicrobial and antioxidant activities than their corresponding ligands.


INTRODUCTION
Various metal complexes with bi-and tridentate Schiff bases containing nitrogen and oxygen donor atoms play important role in biological system [1].Schiff base complexes have numerous applications, such as in the treatment of cancer, as bactericide agent, as antivirus agents, as fungicide agent anti-inflammatory and antipyretic agent in the treatment of diseases such as trypanosomiasis and jaundice and as analytical reagent [2][3][4][5].The importance of metal ion in biological system as macrocyclic compounds is well established because of their catalytic behavior in a number of redox reactions of biological significance [6].2-Hydroxy-1naphthaldehyde Schiff base complexes are widely studied for its antimicrobial, catalytic and redox properties [7].These results have led us to derive Schiff bases of 2-Hydroxyl-1-naphthaldehyde and its Sn (II) complexes.These complexes derived from 2-Hydroxyl-1naphthaldehyde were studied in detail for their applications in diverse areas such as antimicrobial and antioxidant activities.

EXPERIMENTAL Preparation of ligands and complexes
Ligands and complexes were prepared according to the available methods in literature [1,8].Their physical state, melting points, colour, and yield of ligands and complexes are shown in Table 1.

Preparation of HNPA
Equimolar ethanolic solution of 2-Hydroxy-1naphthaldehyde (HN) 0.01 mole (1.7218 g) with 0.01 mole (1.0814g) of o-phenylenediamine (PA) were refluxed with constant stirring for 40 min.The precipitate formed were separated by sintered glass crucible which were dried in vacuum oven.The ligand was reprecipitated with ethanol.

Preparation of HNPT
Equimolar ethanolic solution of 2-Hydroxy-1naphthaldehyde (HN) 0.01 mole (1.7218 g) with 0.01 mole (1.079g) of p-Toludine (PT) were refluxed with constant stirring for 40 min.The precipitate formed was separated by sintered glass crucible which was dried in vacuum oven.

Preparation of HNOT
Equimolar ethanolic solution of 2-Hydroxy-1naphthaldehyde (HN) 0.01 mole (1.7218 g) with 0.01 mole (1.0814 g) of o-Toludine (OT) were refluxed with constant stirring for 40 min.The precipitate formed was separated by sintered glass crucible which was dried in vacuum oven.

Preparation of complexes
Equimolar 0.0025mole ethanolic solution of ligands (HNPA, HNCA, HNPT, and HNOT) and 0.0025mole SnCl 2 .2H 2 O were refluxed with constant stirring for 2 h.The precipitate formed was separated by filtering through sintered glass crucible washed with ethanol for the removal of non-reacted material afterwards samples were dried in vacuum oven.

Antibacterial assay
All synthesized Schiff bases and their Tin (II) complexes were screened in vitro for their antibacterial activity against Gram-negative (Klebsiella pneumoniae and Escherichia coli) and Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis) bacterial strains by the agar-well diffusion method [9].

Antifungal assay
Synthesized Schiff bases and their Tin (II) complexes were investigated for antifungal activity against three fungal strains, i.e.Aspergillus niger, Aspergillus flavus and Alternaria solani by using the agar tube dilution method [10].Nutrient agar was used for culturing of fungal strains.Nutrient agar media (28 g/1000 mL) was prepared in dH 2 O and sterilized in an autoclave for 15 min at 121 o C and 1.5 pounds pressure.

Determination of antioxidant activity
Antioxidant activities of the synthesized ligands, complexes as well as of the standard used were determined by the method of 1, 1-diphenyl-2picryl-hydrazyl (DPPH) free radical activity [11].

Spectroscopic studies
FTIR spectral bands of the relevant ligands and their tin complexes are given in Table 2.
In ligand HNPA and its complex [Sn(HNPA)Cl 2 (H 2 O) 2 ], the υ C=N bands of free ligand at 1600 cm -1 are shifted to higher wave numbers (1618,1653 cm -1 ) in the spectra of the complex, indicating coordination of ligand to the tin ion via the azomethine nitrogen atom.The bands at 3471 and 3373 cm -1 are un-shifted in complex, indicating the free N-H bond in complex.A new band in IR of complex appeared in the region of 561 cm -1 , indicating the Sn-O bond in complex.In ligand HNCA and its complex [Sn(HNCA)Cl 2 (H 2 O) 2 ], the υ C=N bands of free ligand at 1604 and 1558 cm -1 are shifted to higher wave numbers (1618,1593 cm -1 ) in the spectra of the complex, indicating coordination of ligand to the tin ion via the azomethine nitrogen atom.A band at 3028 cm -1 for hydrogen bond by OH group is shifted to 3061 cm -1 , indicating the deprotonation of -OH and its involvement in coordination with metal ion.A new band in IR of complex appeared in the region of 537 cm -1 , indicating the Sn-O bond in complex.In ligand HNPT and its complex [Sn(HNPT)Cl 2 (H 2 O) 2 ] the υ C=N bands of free ligand at 1618 and 1581 cm -1 are shifted to higher wave numbers (1622,1593 cm -1 ) in the spectra of the complex, indicating coordination of ligand to the tin ion via the azomethine nitrogen atom.A band at 3028 cm -1 for hydrogen bonded OH group is shifted to 3061 cm -1 , indicating the deprotonation of O-H and its involvement in coordination with metal ion.A new band in IR of complex appeared in the region of 536 cm -1 , indicating the Sn-O bond in complex.In ligand HNOT and its complex [Sn(HNOT)Cl 2 (H 2 O) 2 ], the υ C=N bands of free ligand at 1610 cm -1 are shifted to higher wave numbers (1622 cm -1 ) in the spectra of the complex, indicating coordination of ligand to the tin ion via the azomethine nitrogen atom.A new band in IR of complex appeared in the region of 543 cm -1 , indicating the Sn-O bond in complex.DMSO-d6 used as solvent for Proton NMR study.The ligand "exhibited signals that have been identified from the integration curve and found to be equivalent to the total number of protons deduced from their proposed structures.These were compared with the reported signals of known identical compounds data".The 1 HNMR data of the four Schiff bases studied are summarized in Table 3.
The phenolic hydroxyl proton in all structures appears in the range of 15-16 ppm.The azomethine protons appear in the range of 8.4 -9.6 ppm which is characteristics of these protons.Signals in the region 7.0 -7.9 ppm were assigned to the aromatic protons.) Table 3: showing the coordination of oxygen with tin metal without the deprotonation of the -OH group.In the case of [Sn(HNPA)Cl 2 ] complex the -OH signal disappears indicating the deprotonation of oxygen atoms of the ligand on complexation.The multiplets between 6.8 -8.5 ppm in the spectra of ligands are assigned to the naphthylidene group protons.These multiplets have downfield shift in the spectra of the complexes attributed to increased conjugation upon complex formation.In all the complexes new peak appear in the region of 3.0-3.4ppm were assigned for coordinated water 13 C NMR Spectra of the four ligands were recorded in DMSO-d6 and these spectra also support the authenticity of the proposed structures (Table 4).
The signals observed at 167-171.8 ppm are due to azomethine carbons.The peak for the -OH group appears at 154.5-156.6 ppm.The aromatic carbon directly attached with azomethine carbon shows peak at 39.2-39.7 ppm.The aromatic carbon directly attached with azomethine nitrogen shows peak at 141 -143.3 ppm.The methyl carbons in the ligands HNPT and HNOT appears in the range of 17.7 -20.5 ppm.The carbon having chlorine atom in l-2 showed signal at 130.63 ppm.Carbon attached with the unreacted -NH 2 group shows signal at 141.76 ppm.All other aromatic carbons showed peak in the range of 121 -133 ppm.In the  5.The atomic absorption data for tin metal is also shown in Table 5.The data establishing the general formula of complexes is [Sn(L)Cl 2. nH 2 O] "where L is ligand, and n=1 or 2, is the number of coordinated water".

Molar conductance
In Table 6 the molar conductance data of synthesized complexes are given which indicate that all of them are non-electrolytic in nature in the solvent.Because in electrolytic complexes the anion bonded outside the coordination sphere with metal.Also low conductance results support the reality that the "Schiff bases are coordinated to the tin atom in their deprotonated anionic forms" and that the two chlorides ligands are also coordinated to the tin atom.

Electronic spectra
The electronic absorption spectra of ligands and their Sn (II) complexes in DMF solution were carried out in the range of 200-1000 nm at room temperature (Table 7).
There is a shift of the bands to longer λ in spectra of all Sn (II) complexes is a good evidence of complex formation.The ligand HNPA chromophore HC=N which absorbs at 270 nm, due to n-п* transition of azomethine group.The shift to the longer wavelength at 310 nm in the spectra of tin (II) complex [Sn(HNPA)Cl 2 (H 2 O) 2 ] is a good evidence of complex formation and coordination of azomethine nitrogen in the complex.The complex absorption showed two sharp bands at 340 nm and 360 nm and these may be assigned as charge transfer bands.It has been reported that the metal is capable of forming dп-pп* bonds with ligands containing nitrogen as the donor atom.The Tin atom has its 5d orbital completely vacant and hence Sn-N bonding can take place by the acceptance of the lone pair of electrons from the nitrogen of the ligands.In the electronic spectra of the ligand HNCA, a band at 220 nm was observed which may be assigned to 1B band of phenyl ring.The electronic spectra of the ligand consist of a band at 240 nm have been assigned θ-θ* transitions within the benzenoid ring.This band remains almost unchanged in the electronic spectra of the complex [Sn(HNCA)Cl 2 (H 2 O) 2 ].The UV/Vis spectrum of the ligand HNPT showed three bands.One is at 360 nm which is assigned to nπ* transition.Second band at 400 nm is due to nπ* electronic transitions of azomethine.In Sn (II) complex [Sn(HNPT)Cl 2 (H 2 O) 2 ] a band appears at 390 nm is possibly due to the polarization in C=N bond caused by the metal-ligand electronic interaction.The electronic spectra of the free ligand HNOT, the bands recorded at 320 nm and 360 nm are assigned to n-π* transitions of azomethine group.These bands shifted to higher wavelength on coordination through azomethine nitrogen in the complex [Sn(HNOT)Cl 2 (H 2 O) 2 ].
The band at 310 nm are due to π-π* transitions within the azomethine.The band at 370 nm in complex is possibly due to polarization of the C=N bond caused by the metal-ligand interaction.

Antimicrobial activity
The antimicrobial activities results clearly showed that the synthesized ligands and their tin comp lexes are biological active as shown in Table 8 and Table 9.From the data obtained, it is evident that some of these complexes exhibited significant activity (p < 0.05) against the tested organisms.

Antioxidant activity
Significant antioxidant potential was recorded for ligands and their Tin (II) complexes.Antioxidant activity for complexes was active EC 50 values are given in Table 10.Lower EC 50 value showed higher antioxidant potential of all the tested samples.

DISCUSSION
Schiff base complexes catch researcher's interest due to its significant role in biologically important species and in the bioinorganic chemistry development [12,13].The carbon having chlorine atom in l-2 showed signal at 130.63 ppm.Carbon attached with the unreacted -NH 2 group shows signal at 141.76 ppm.All other aromatic carbons showed peak in the range of 121 -133ppm.In the 13 C-NMR spectra of the ligands, the signals observed in the range of 167 -171.8 ppm are due to azomethine carbons.These signals shift (δ 168.9 -169.4 ppm) in the case of tin (II) complexes, indicating the coordination of azomethine nitrogen to the tin [14].
Low conductance data also support the fact that the Schiff bases are coordinated to the tin atom in their deprotonated anionic forms and that the two chlorides ligands are also coordinated to the tin atom [15].The tin atom has its 5d orbital completely vacant and hence Sn-N bonding can take place by the acceptance of the lone pair of electrons from the nitrogen of the ligands [16].In the electronic spectra of the ligand HNCA, a band at 220 nm was observed which may be assigned to 1B band of phenyl ring [17].
Several Schiff base metal complexes were investigated for antibacterial assessment and revealed that metal complexes are more active as compared to free ligands [18].Antioxidant activity for complexes was active at various EC 50 values.Lower EC 50 value was attributed to higher antioxidant potential of all the tested samples [19].

CONCLUSION
The complexes synthesized from 2-Hydroxyl-1naphthaldehyde exhibit various biological activities.They possess potentials that can be further developed to exert strong antimicrobial and antioxidant activities.
Trop J Pharm Res, December 2016; 15(12): 2700 13C-NMR spectra of the ligands, the signals observed in the range of167 -171.8  ppm are due to azomethine carbons.These signals shift (δ 168.9 -169.4 ppm) in the case of Tin (II) complexes, indicating the coordination of azomethine nitrogen to the tin.Signals in the range of δ 154 -157.4 ppm are assigned to the aromatic carbons attached to the -OH group in various ligands.The shifts were observed in these signals (δ 154-159.3ppm) when the free ligands are coordinated to the tin atom indicating the involvement of -OH group in complex formation.In the 13 C-NMR spectra of the ligands, the signals observed in the range of δ 141.08 -143.38 ppm are due to aromatic carbons attached to azomethine nitrogen.The shift in these signal are observed in the range of δ 141.10 -157.41 ppm.Elemental analysis and atomic absorption data"The close agreement in the calculated and found values of the elemental analysis" shows the formation of ligands (HNPA,HNCA, HNPT and HNOT) and complexes ([Sn(HNPA)Cl 2 (H 2 O) 2 ], [Sn(HNCA)Cl 2 (H 2 O) 2 ], [Sn(HNPT)Cl 2 (H 2 O) 2 ] and [Sn(HNOT)Cl 2 (H 2 O) 2 ]) as seen in Table

Table 1 :
Physical properties of the ligands and its complexes

Table 2 :
FTIR data for ligands and its complexes Three protons of the methyl group in the ligands HNPT and HNOT appear in the range of 2.3 -2.4 ppm.Signal of the two protons of NH 2 -group in the ligand HNPA appears at 5.12 ppm.The signals for azomethine proton appear in the range of 9.61-9.94ppm for ligands shift downfield in the spectra of Tin (II) complexes.

Table 5 :
Elemental analysis and atomic absorption data for ligands and complexes

Table 6 :
Molar conductance analysis of complexes

Table 7 :
Electronic spectral data of complexes

Table 8 :
Antibacterial activity of ligands and its Tin (II) complexes

Table 9 :
Antifungal activity of ligands and their Tin (II) complexes

Table 10 :
Antioxidant activity of ligands and its Tin (II) complexes