Rhodiola rosea L extract shows protective activity against Alzheimer ’ s disease in 3 xTg-AD mice

Purpose: To investigate the protective effect of Rhodiola rosea L. extract (RRLE) against Alzheimer's disease in 3xTg-AD mice. Methods: The cognitive function of 3xTg-AD mice was assessed using Morris water maze test. The levels of amyloid beta deposits and NeuN in the hippocampus were evaluated by immunohistochemistry. Brain neurotrophic-derived factor (BDNF) and tyrosine kinase B (TrkB) expressions were examined by western blot analysis. Results: RRLE treatment significantly ameliorated learning and memory deficits in AD mice, as shown by increased time spent in the target zone during probe tests. The escape latency in animals treated with 400 mg/kg RRLE (20.5 ± 1.3 s) was significantly increased compared to the untreated mice (12.4 ± 1.3 s, p < 0.01). In addition, RRLE significantly decreased Aβ deposits, increased NeuN-positive cells, and upregulated the expression of BDNF (1.4 ± 0.2, p < 0.05) and TrkB (1.1 ± 0.2, p < 0.05) in the mice. Conclusion: The findings suggest that RRLE treatment may be a useful strategy for treating memory impairment induced by several neurodegenerative diseases.


INTRODUCTION
Alzheimer's disease (AD) is the most common form of dementia in the elderly and accounts for between 50 and 75 % of all cases.By 2030 it is estimated that more than 65 million people will be living with dementia, with projections almost doubling every 20 years.AD is a progressive neurodegenerative disorder characterized, at least in part, by abnormal accumulation of βamyloid peptide (Aβ) in the brain [1].The accumulated Aβ is believed to play an important role in the pathogenesis of AD [2].Thus, Aβ continues to be an important target for prevention and treatment of AD [3].
Alzheimer's disease is the most common form of dementia relating to memory and cognitive decline.AD is a progressive neurodegenerative disease in which dementia symptoms gradually worsen over a number of years [4].Classical biochemical hallmarks of AD include the accumulation of amyloid beta (Aβ) peptide oligomers and soluble hyperphosphorylated tau [5].Brain-derived neurotrophic factor (BDNF) is a small dimeric protein, and BDNF acts through high affinity binding with its receptor, tyrosine kinase B (TrkB).BDNF modulates neuronal growth and survival, and BDNF is implicated in learning and memory processes; therefore, dysfunction in BDNF is accompanied by cognitive deficits.In particular, BDNF is involved in the AD-related decline of neurogenesis, and levels of nerve growth factor are also diminished with AD [6].
Medical treatment for AD patients is placing an increasing burden on physicians and families every year.Clinically, there are a variety of drugs available for AD, such as cholinesterase inhibitors, glutamate receptor antagonists, and free radical scavengers.However, these drugs do not closely target the pathogenesis of the disease and have numerous side effects [7].Therefore, it is extremely important to elucidate the mechanism of AD pathophysiology to find a new drug for treatment.
Rhodiola rosea L., has been used in China in the treatment of various disorders, including stressinduced physiological changes, inflammation, hypertension, and cancer.Rhodiola rosea L. extract has been reported to have anti-oxidant [8], immunomodulatory [9], and anti-mutagenic activity [10].In this study, we investigated to evaluate the neuroprotective effects of RRLE on learning and memory deficits in a tripletransgenic mouse model of Alzheimer's disease (3xTg-AD), which expresses APP Swe , PS1 M146V , and tau P301L [11].

EXPERIMENTAL Plant material
The plant material, Rhodiola rosea L., were collected from Pingdingshan City, Henan Province in China in October 2015.Taxonomic identification of the plant was performed by Professor Gang Wang of Shandong University, in China.A voucher specimen (no.PRLE 201605002) was deposited in College of Pharmacy, Shandong University, China for future reference.
The herbal samples Rhodiola rosea L. was dried in an oven.RRLE was obtained by steeping the dried Rhodiola rosea L. in water at 60 o C three times, each for one hour before first drying in an oven and then freeze-drying the RRLE thus obtained.One gram powder was equivalent to about 1.7 g crude samples.Thus, the yield was 58.82 %.

Animals
Alzheimer's disease (3xTg-AD) mice carrying a mutant APP (KM670/671NL), a human mutant PS1 (M146V) knock-in, and tau (P301L) transgenes (B6;129-Psen1 tm1Mpm Tg (APPSwe,tauP301L)1Lfa/J) were purchased from the animal research institute, Nanjing University (Nanjing, China).The non-transgenic littermates were used as wild type (WT) controls.All animals were kept in a pathogen-free environment on a 12 h light/dark cycle and had access to food and water ad libitum.

Animal groups
The mice were randomly divided into four groups as follows: (1) [12].

Water maze test
A modified version of the water maze procedure described by Morris was used to test each mouse's cognitive function [13].The water maze was a circular pool 0.9 m in diameter and constructed of fiberglass.Water in the pool was maintained at 22 ± 2 o C and mixed with 1 kg of powdered skim milk to make the water opaque.
During testing in the water maze, a platform (6 cm in diameter) was fixed 1 cm below the surface of the water at identical location within the pool.The pool was surrounded by differing extra-maze cues.All mice were subjected to four trials per day at intervals of 15 min for four consecutive days.The proportion of time spent searching for the platform in the training quadrant, i.e., the previous location of the platform, was used as a measure of memory retention.

Western blotting
At the end of the experiment, the mice were decapitated and the brains were rapidly removed and placed on ice.

Densitometry analysis
Coronal sections of the hippocampus were examined from the rostral anteroposterior (-2.1 mm) to the anteroposterior (-4.5 mm) direction, as defined by the bregma of the brain atlas.Images were obtained at 10 × magnification using the IMAGE PRO PLUS System (version 4.0; Media Cybernetics, Silver Spring, MD, USA) on a computer attached to a light microscope (Zeiss Axioskop, Oberkochen, Germany), which interfaced with a chargecoupled device video camera (Kodak Mega Plus model 1.4 I).To determine the density of the Aβ-immunoreactive staining in the hippocampus, a square frame of 500 × 500 μm 2 was placed in the dorsal part of the hippocampus.A second square frame of 200 × 200 μm 2 was placed in the corpus callosum to measure the background.As previously described, variations in background illumination were controlled by subtracting the average background density of the corpus callosum from the average density of the hippocampus in each section analyzed [14].

Statistical analysis
Data are presented as mean ± standard deviation (SD).Results were analyzed statistically by one-way ANOVA followed by Tukey's multiple comparison using SPSS 16.0 software for Windows.Differences were considered statistically significant at p < 0.05.

RRLE reverses spatial learning deficits at 6 months of age in mice
To examine spatial memory, the performance of animals in the probe trial was compared to the time animals spent swimming to the platform.All animals were examined by a retention test that involved removing the platform from the pool on the fourth trial day.From Table 1, the escape latency in animals treated with 400 mg/kg RRLE was significantly increased as compared to the 3xTg-AD mice.3xTg-AD mice displayed severely impaired spatial cognition in the water maze test compared with the WT controls, and the administration of RRLE ameliorated these learning and memory deficits.

Effect of RRLE on Aβ pathology in mice
In 3xTg-AD mice, Aβ is present in the hippocampus by 6 months of age.Therefore, to investigate the link between neurogenesis and the development of AD pathology, we assessed the Aβ burden in brains from 3xTg-AD mice that began receiving RRLE treatments 3 month prior to 6 months of age.Aβ deposits in the CA1 region of the hippocampus were significantly increased in 6-month-old 3xTg animals compared to the age-matched WT group (p < 0.01).In the CA3 region of the hippocampus, Aβ deposits were significantly increased in the 3xTg group (p < 0.001) compared to the WT group (p < 0.01).
Compared with the 3xTg group, Aβ deposits in the CA1 region were significantly decreased by RRLE 400 mg treatment (p < 0.05).In addition, compared with the 3xTg group, Aβ deposition in the CA3 was significantly decreased by RRLE 200 mg treatment (p < 0.05).However, there were no significant differences between the 3xTg group and the 3xTgþ RRLE (400 mg/kg) (Table 2).

Effect of RRLE on the expressions of BDNF and TrkB and in mice hippocampus
The effect of RRLE treatment on the expression of hippocampal BDNF and TrkB in mice was determined.The results are shown in Table 3.The expression of hippocampal BDNF and TrkB of mice treatment with saline was significantly lower compared to that of WT mice (p < 0.05).It was also demonstrated that RRLE treatment significantly increased the expression of hippocampal BDNF and TrkB.These results show that the induction of AD reduced BDNF and TrkB expressions in the hippocampus, whereas, RRLE treatment enhanced BDNF and TrkB expressions in the hippocampus of the 3xTg mice.

DISCUSSION
The present study demonstrated that RRLE increased spatial learning, memory abilities, and the expression of hippocampal BDNF and TrkB in 3xTg-AD mice.In the present study, we chose to use 3xTg-AD mice, a model derived from APPS we , PS1 M146V , and tau P301L transgenes.The 3xTg-AD mice develop a progressive, agerelated neuropathological phenotype that includes plaque and tangle pathologies.Both of these hallmark lesions are mainly limited to the hippocampus, amygdala, and cerebral cortexthe brain structures most impacted by AD pathology [15].
Cognitive impairment has been detected in 2month-old 3xTg-AD mice [16], and Aβ deposits in the hippocampus and cortex have been found in 6-month-old 3xTg-AD mice [17][18][19].These findings indicate that the pathological features that imitate AD in 3xTgAD mice remain stable.Classic symptoms of AD include problems with spatial learning and memory deficits.Our study demonstrated that treatment with RRLE resulted in a significant restoration of spatial learning and memory function in AD mice.These results suggest that RRLE treatment may be effective in ameliorating cognitive impairment caused by AD.
Neuron-specific nuclear antigen (NeuN) is a neuronal-specific nuclear protein [20,21].The expression of NeuN is observed in most neuronal cell types throughout the nervous system, with the exception of some neuronal populations that are NeuN-negative, but does not stain nonneuronal cells [22].NeuN is a soluble nuclear protein that is localized to the cell nucleus and in the neuronal cytoplasm of postmitotic neurons.Within the hippocampus, NeuN can be used as a marker of postmitotic cells and labels both "normal" postmitotic neurons and newly generated postmitoticneurons.BDNF plays pivotal roles in learning, memory, and neuronal plasticity.
The levels of BDNF, and its main receptor TrkB, have been reported to lessen in AD.It was hypothesized that BDNF and its receptor may be involved in the protective role of RRLE against memory impairment.Results of the present study have demonstrated that RRLE intake significantly increases the expression of BDNF and its main receptor TrkB, in the brain, which is in agreement with our hypothesis.There is evidence to support our results.It has been demonstrated that BDNF and TrkB, are capable of protecting against memory impairment and regulate neurogenesis in the hippocampus of AD.A recent study also supports the role of BDNF signaling through TrkB in the pathophysiology and cognitive and its receptor involving RRLE in AD [23].

CONCLUSION
The findings of this study reveal that RRLE attenuates learning and memory deficits in 3xTg-AD mice, and thus, the plant material can potentially be developed as an alternative therapeutic agent for AD.

Table 1 :
Effect of RRLE on behavior in a Morris water maze

Table 2 :
Effect of RRLE on the deposition of Aβ in mice hippocampus

Table 3 :
Effect of RRLE on BDNF and TrkB expression in mice hippocampus