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Published:
Dec 8, 2015Keywords:
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Main Article Content
Effect of the Extract of Endophytic fungus, Nigrospora sphaerica CL-OP 30, Against the Growth of Methicillin- Resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Klebsiella pneumonia</i> cells
Abstract
Purpose: To investigate the effect of the ethyl acetate extract of endophytic fungus, Nigrospora sphaerica CL-OP 30 against the growth of methicillin-resistant Staphylococcus aureus (MRSA) and Klebsiella pneumonia cells.
Methods: Time-kill assay was used to examine the effect of the extract on the bacterial growth profile. The effects of extract on ultrastructure of MRSA and K. pneumoniae cells were analyzed by scanning electron microscope (SEM) and transmission electron microscope (TEM).
Results: The time-kill test revealed that the bacteriocidal activity of the extract was both concentrationand time-dependent. After 12 h treatment, interaction of extract with MRSA cells resulted in the formation of pit, disintegration of cell wall and membrane, and ultimately cell death, while K. pneumoniae cells became crumpled, and the cell walls and membranes disintegrated, resulting in leakage of their cytoplasmic contents.
Conclusion: These data suggest that the Nigrospora sphaerica CL-OP 30 extract principally affects the cell wall in growing MRSA and K. pneumonia cells.
Keywords: Endophytic fungus, Nigrospora sphaerica, Antimicrobial activity, Cellular structure degeneration
