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Anti-biofilm and antimicrobial activity of <i>Mentha pulegium</i> L essential oil against multidrug-resistant <i>Acinetobacter baumannii</i>


Uğur Tutar
Cem Çelik
İsa Karaman
Mehmet Ataş
Ceylan Hepokur

Abstract

Purpose: To investigate the antimicrobial and anti-biofilm activities of essential oil from Mentha pulegieum L. (EOMP) on multi-drug resistant (MDR) isolates of A. baumannii, as well as its phytochemical composition, antioxidant properties and cytotoxic activity.

Methods: The phytochemical composition of EOMP was analyzed by gas chromatography, while its antimicrobial activities were determined by disc diffusion and broth micro-dilution methods. Minimal biofilm inhibition concentration (MBIC) and minimal biofilm eradication concentration (MBEC) tests were used for assessment of its anti-biofilm properties. Viability in the biofilm was studied using 2,3-bis (2- methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay, while colorimetric assay was used to assess its cytotoxicity on L929 cells

Results: D-isomenthone, pulegone, isopulegone, menthol and piperitenone were the major components of the plant extract. EOMP produced > 22 mm inhibition zone for the isolates, with minimum inhibitory concentration (MIC) and MBIC of 0.6 - 2.5 and 0.6 - 1.25 μL/mL, respectively, while MBEC was ≥ 10 μL/msL. EOMP damaged biofilm structures formed by A. baumannii strains at MIC by 26 – 91 %.

Conclusion: These results suggest that EOMP contains agents that may be useful in the development of new drugs against A. baumannii infections.

Keywords: Mentha pulegium, Essential oil, Anti-biofilm, Antimicrobial activity, Multi-drug resistance, A. baumannii


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eISSN: 1596-9827
print ISSN: 1596-5996