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Published:
Mar 16, 2019Keywords:
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Main Article Content
Allicin-induced modulation of angiogenesis in lung cancer cells (A549)
Abstract
Methods: Allicin solution at different concentrations was tested on A549 lung cancer cell line. Viability and proliferation of A549 cells were determined. The cytotoxic effects of allicin solution on A549 lung cancer cells were quantitatively determined using Alamar blue assay. Cell proliferation and cell cycle were measured by DAPI-flow cytometry analysis in order to investigate the possible cell signalling pathway targeted by allicin. The level of expression of VEGF-A protein was determined with confocal laser scanning microscopy.
Results: Allicin inhibited lung cancer proliferation and down-regulated the protein expression of VEGF, but had no significant cytotoxic effects on A549 cells. Flow cytometric results showed that allicin induced cell cycle arrest of A549 cells at the G1 phase.
Conclusion: These results indicate that allicin exerts anti-proliferative effects on A549 lung cancer cells. Thus allicin, an active component of garlic, might be a promising therapy against lung cancer metastasis.
Keywords: A549 cells, Allicin, qPCR Taqman assay, VEGF-A protein expression, Garlic, Lung cancer
