Article Sidebar
Published:
Mar 16, 2019Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Antiplasmodial and onset speed of growth inhibitory activities of <i>Tithonia diversifolia</i> (Hemsley) A Gray leaf fractions against <i>Plasmodium falciparum</i>
Abstract
Methods: Seven fractions of T. diversifolia (F1-F7) were used in this study. Phytochemical analysis was conducted to identify the major compounds in the fractions. Various concentrations of fractions ranging from 2.5 – 100.0 μg/mL were exposed to P. falciparum FCR3 strain for 60 h and the growth inhibition was then calculated. The fraction which exhibited the best antiplasmodial activity was tested further to determine the growth inhibition onset against P. falciparum FCR3 strain. This was achieved by examining the inhibitory activity of the fraction when it was added at the beginning of the experiment and assessing subsequent parasite growth after 8, 16, 24, 32, and 40 h incubation.
Results: The major compounds found in the fractions were terpenes. Fraction six (F6) had the best antiplasmodial activity (IC50 13.63 ± 1.43 μg/mL). During the first 32 h of incubation, F6 inhibited the growth of parasites and this increased with longer incubation time; 32 h incubation provided the highest growth inhibition (99.23 ± 0.05 %). After 32 h the inhibition activity began to decrease, and resulted in < 50 % inhibition at 48 h incubation. This result suggested that F6 is a rapid-onset antiplasmodial agent.
Conclusion: Fractions of T. diversifolia, especially F6, are promising antimalarial agents and require further development for clinical application.
Keywords: Tithonia diversifolia, Plasmodium falciparum, Onset speed, Antimalarial, Antiplasmodial
