Article Sidebar
Published:
Oct 29, 2013DOI:
10.4314/tjpr.v12i5.7Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Methanol Extract of <i>Codonopsis pilosula</i> Inhibits Inducible Nitric Oxide Synthase and Protein Oxidation in Lipopolysaccharide-Stimulated Raw Cells
Abstract
Purpose: To evaluate the mechanism of antioxidant activity of the methanol extract of Codonopsis pilosula.
Methods: Anti-oxidative properties were assessed by measuring free radical scavenging activity, nitric oxide (NO) levels, protein oxidation and reducing power, while the mechanism of antioxidative effect of Codonopsis pilosula extract was determined by measuring iNOS and COX-2 expression in lipopolysaccharide (LPS)-stimulated raw cells.
Results: Codonopsis pilosula extract (CPE) exerted significant DPPH free radical and NO-scavenging activities. Protein oxidation was decreased by 30 % by the CPE (1mg/ml). The extract (1mg/ml) enhanced reducing power 16-fold (compared with control). LPS-induced production of iNOS was significantly inhibited by the extract (60 %), suggesting that it inhibits NO production by suppressing iNOS expression. However, LPS-induced production of COX-2 was not significantly (p < 0.05) inhibited by the extract. The levels of total phenolics, total flavonoids, and total anthocyanin in CPE were 0.38, 2.10, and 2.47 mg/g, respectively.
Conclusion: These results suggest that CPE exerts marked antioxidant activity via inhibition of iNOS and protein oxidation.
Keywords: Codonopsis pilosula, Lipopolysaccharide, Cyclooxygenase-2 (COX-2), Free radicals, Inducible nitric oxide synthase (iNOS), Nitric oxide (NO)
