PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Tyrosinase Inhibitory Activity and Thermostability of the Flavonoid Complex from Sophora japonica L (Fabaceae)

JS Lai, C Lin, TM Chiang

Abstract


Purpose: To investigate the tyrosinase inhibitory activity and thermostability of weak acid-treated Sophora japonica L. flavonoid complex (SJ-FC) in different solutions.
Methods: The flavonoid complex of S. japonica was isolated and treated with weak acid to generate SJ-FC. The anti-tyrosinase activities of SJ-FC and well-known tyrosinase inhibitors were compared by mushroom tyrosinase activity assay. The thermostabilities of SJ-FC and other inhibitors in differentsolution environments for long-term storage were also investigated.
Results: The results indicate that SJ-FC has potent tyrosinase inhibitory activity, and at a concentration of 0.1 %, SJ-DC has a tyrosinase inhibitory activity equal to that of 1 % ascorbic acid or hydroquinone. In addition, SJ-FC in both propylene glycol (PG) and glycerol solutions exhibited obvious tyrosinase inhibitory activity. Ascorbic acid and arbutin, two other tyrosinase inhibitors, exhibit < 60 % of their initial activity in both PG and H2O solutions after 6 months of storage. However, SJ-FC stored in PG and H2O solutions retained almost 100 % of its activity over a 6-month period.
Conclusion: SJ-FC is an effective and stable anti-tyrosinase agent and may be used as a function agent in medicines, foods and cosmetics.

Keywords: Flavonoid, Sophora japonica L., Tyrosinase inhibitor, Thermostability




http://dx.doi.org/10.4314/tjpr.v13i2.12
AJOL African Journals Online