In vitro Antimicrobial and Antibiofilm Activity of Artocarpus Lakoocha (Moraceae) Extract against Some Oral Pathogens

  • R Teanpaisan
  • S Senapong
  • J Puripattanavong
Keywords: Artocarpus lakoocha, Antimicrobial, Biofilm, Dental caries, Periodontal diseases, Time-kill assay


Purpose: To evaluate the antimicrobial and antibiofilm activity of A. lakoocha extract against oral pathogens by an in vitro method.
Methods: The dried powder of the aqueous extract of A. lakoocha was purchased from a Thai traditional drug store. Representative strains of oral pathogens (Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 33478, Enterococcus faecalis ATCC 19433, Lactobacillus fermentum ATCC 14931, Lactobacillus salivarius ATCC 11741, Aggregatibacter actinomycetemcomitans ATCC 33384, Porphyromonas gingivalis ATCC 33277, Prevotella intermedia ATCC 25611, Prevotella nigrescens ATCC 25261, Fusobacterium nucleatum ATCC 25586 and Tanerella forsythia ATCC 43037) were tested for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using a microdilution technique, as well as by a time kill assay. Antibiofilm activity was investigated by a 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay.
Results: All tested strains were susceptible to A. lakoocha extract with variable degrees of antimicrobial inhibition. The extract was effective against both Gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and Gram-positive bacteria (Streptococcus mutans, Streptococcus sobrinus), with MIC ranging from 0.10 – 0.39 mg/ml and MBC from 0.10 – 3.12 mg/ml. Killing activity depended on time and concentrations of the extract. The extract acted as a potent antibiofilm agent with dual actions, preventing biofilm formation and also eradicating the existing biofilm.
Conclusion: A. lakoocha extract possesses compounds with good antimicrobial properties that may be used for oral infectious diseases caused by certain oral pathogens associated with dental caries and/or periodontal diseases. For the application, A. lakoocha extract may be incorporated in mouthwash or toothpaste.

Keywords: Artocarpus lakoocha, Antimicrobial, Biofilm, Dental caries, Periodontal diseases, Time-kill assay


Journal Identifiers

eISSN: 1596-9827
print ISSN: 1596-5996