Protein expression of Myt272-3 recombinant clone and in silico prediction of a possible vaccine candidate against Mycobacterium tuberculosis

  • Mohammed Maikudi Usman
  • Salmah Ismail
  • Teow Chong Teoh
Keywords: Vaccine, Mycobacterium tuberculosis, Epitope prediction, Histocompatibility complexes

Abstract

Purpose: To investigate the expression of Myt272-3 recombinant protein and also to predict a possible protein vaccine candidate against Mycobacterium tuberculosis.
Methods: Myt272-3 protein was expressed in pET30a+-Myt272-3 clone. The purity of the protein was determined using Dynabeads® His-Tag Isolation & Pulldown. Protein sequence was analysed in silico using bioinformatics software for the prediction of allergenicity, antigenicity, MHC-I and MHC-II binding, and B-cell epitope binding.
Results: The candidate protein was a non-allergen with 15.19 % positive predictive value. It was also predicted to be antigenic, with binding affinity to MHC-I and MHC-II, as well as B-cell epitope binding.
Conclusion: The predicted results obtained in this study provide a guide for practical design of a new tuberculosis vaccine.


Keywords: Vaccine, Mycobacterium tuberculosis, Epitope prediction, Histocompatibility complexes

Published
2016-08-11
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996