Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items
Purpose: To study the vacA genotype status of H. pylori isolated from some Iranian food items.
Methods: Three hundred assorted samples of fish, ham, chicken, vegetable and meat sandwiches, and minced meat were purchased and tested using culture method. Those that were H. pylori-positive were analyzed for presence of vacA genotypes using polymerase chain reaction (PCR).
Results: Sixty out of 300 (20 %) food samples were positive for H. pylori. Vegetable sandwich (45 %), minced meat (32 %) and meat sandwich (20 %) were the most commonly contaminated. The most commonly detected genotypes in the meat-based foods, viz, vegetable sandwich and ready to eat fish, were vacA s1a, vacA m1a and vacA m2, respectively. The most commonly detected combined genotypes were s1am2 (45 %), s1am1a (40 %) and m1am2 (35 %).
Conclusion: The presence of similar genotypes in H. pylori strains of foods and those of human clinical samples suggest that contaminated foods may be the source of bacteria for humans.
Keywords: Helicobacter pylori, VacA genotypes, Genotyping, Food items
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.