Anti-tumor effect of polysaccharides from rhizome of Curculigo orchioides Gaertn on cervical cancer
Purpose: To investigate the anti-tumor effects of polysaccharides from Curculigo orchioides (PDC) on cervical cancer and the possible mechanisms involved.
Methods: A Box–Behnken design (BBD) was employed to optimize extraction conditions for PDC. The anti-tumor effect of PDC on cervical cancer was investigated in vivo in mice injected with Hela cells. The parameters measured were tumor volume and weight. In vitro anti-tumor effects of PDC were assessed by measuring expressions of caspase-3, caspase-9 and P53 proteins in Hela cells via ELISA assay. Thymus and spleen indices were calculated for assessment of PDC effect on immune function.
Results: The optimum extraction conditions predicted by the response surface methodology (RSM) were: extraction time = 1.58 h, ratio-of-water-to-sample = 30.05 mL/g and extraction number = 1.95. PDC showed significant anti-tumor effect on cervical cancer in mice. It significantly increased thymus and spleen indices in mice; and significantly up-regulated expressions of caspase-3, caspase-9 and P53 proteins in HeLa cells.
Conclusion: PDC has significant anti-tumor effect on cervical cancer in vivo and in vitro, most probably through mechanisms involving enhancement on immune function and induction of apoptosis.
Keyword: Curculigo orchioides, Polysaccharides, Cervical cancer, HeLa cells, Apoptosis