Expression of Oct-4 in oncogenic miR-155-positive oral squamous carcinoma cells

  • Jun-Song Jiang
  • Jia Zhu
  • Hua Han
  • Qing Wang
Keywords: CD44, oral squamous cell carcinoma, oncogenic miR-155, Oct-4

Abstract

Purpose: To identify effective molecular diagnostic methods for oral squamous cell carcinoma (OSCC) to facilitate treatment of the disease in its initial stages.

Methods: To identify molecular markers, OSCC tissue samples were collected from cancer patients and healthy controls. CD44+ cells were sorted using quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry and immunostaining experiments were performed to identify markers for OSCC.

Results: The qRT-PCR data confirmed the presence of oncogenic miR-155 in the OSCC samples. The immunohistochemical and immunostaining results confirmed the expression of Oct-4, an important target for the early diagnosis of OSCC, in oncogenic miR-155-positive OSCCs.

Conclusion: Detection of the expression of miR-155 and Oct-4, which are key molecular markers, may be useful in improving the early diagnosis of OSCC.

Keywords: CD44, oral squamous cell carcinoma, oncogenic miR-155, Oct-4

Published
2016-10-05
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996