Expression profiling of miR-96, miR-584 and miR-422a in colon cancer and their potential involvement in colon cancer pathogenesis

  • Min Lu
  • Xuan Zhou
  • Cheng-Guo Zheng
  • Feng-Jun Liu
Keywords: Colon cancer, miR-96, miR-422a, miR-584, Expression profiling, Biomarker

Abstract

Purpose: To determine the correlation between miRNAs; miR-96, miR-422a and miR584, and colon cancer, and also to test whether any of these miRNAs can act as non-invasive biomarkers in colon cancer

Methods: The tumor samples and the corresponding normal mucosa used in this study were collected from 60 patients diagnosed with colon cancer. HT-29, HCT-116 and SW-620 cell lines were used for miRNA expression profiling. Total RNA was extracted using kit-based methods with minor modifications, and specific miRNAs were detected and quantified by quantitative real-time polymerase chain reaction [RT-PCR].

Results: The results indicate down-regulation of miR-96, miR-584 and miR-422a in colon cancer tissue and decreased expression levels in the three colon cancer cell lines (all p < 0.01). Lower miRNA expression levels are correlated with increased tumor size.

Conclusion: This study shows that miR-96, miR-584 and miR-422a are down-regulated in colon cancer and are associated with tumor size. Thus, the ratio of miR-96/miR-638 in plasma is a potential noninvasive biomarker in colon cancer.

Keywords: Colon cancer, miR-96, miR-422a, miR-584, Expression profiling, Biomarker

Published
2017-01-12
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996