PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Ameliorative effect of Citrus aurantifolia and Cinnamomum burmannii extracts on diabetic complications in a hyperglycemic rat model

Herin Mawarti, Mohammad Zulfikar As’ad Khotimah, Mukhammad Rajin

Abstract


Purpose: To evaluate the effects of Citrus aurantifolia and <i>Cinnamomum burmannii</i> extracts (Jermanis) on the number of pancreatic β-cells and levels of superoxide dismutase (SOD), low-density lipoprotein
(LDL), and transforming growth factor-beta (TGF-β) in β-cells.
Methods: Various doses of a combination of extracts of <i>C. aurantifolia</i> and </i>C. burmannii </i>were given orally to 25 male Wistar rats (<i>Rattus  norvegicus</i>) every 2 h for 30 days. Doses of 100, 300 and 500 mg/kg/day <i>C. aurantifolia</i> extract were considered low, medium and high doses, respectively, while, 200, 400 and 800 mg/kg/day <i>C. burmannii</i> extract were considered low, medium and high doses, respectively. LDL and SOD levels in blood serum were analyzed spectrophotometrically. TGF-β expression was evaluated by immunohistochemistry. Pancreatic tissue sections (diameter of a β-cell) were evaluated by light microscopy after hematoxylin and eosin staining.
Results: The combination of <i>C. aurantifolia</i> and <i>C. burmannii </i>extracts increased SOD levels, TGF-β expression and the number of β-cells and decreased LDL levels in hyperglycemic rats. The results indicate that the highest doses of <i>C. aurantifolia</i> and <i>C. burmannii</i> increased the number of β-cells in the islets of Langerhans. The combined extracts of <i>C. aurantifolia</i> and <i>C. burmannii</i> significantly affected pancreatic cell regeneration.
Conclusion: The combination of <i>C. aurantifolia</i> and <i>C. burmannii</i> extracts may be a promising alternative preventative medicine for management of diabetic complications in patients with hyperglycemia.


Keywords: β-cell, Diabetes, Diabetic complications, C<i>itrus aurantifolia, Cinnamomum burmannii</i>, Biochemical profile, TGF-β expression




http://dx.doi.org/10.4314/tjpr.v17i5.11
AJOL African Journals Online