MicroRNA miR-103a-3p targets NPAS3 to regulate progression of Alzheimer’s disease
Purpose: This study aimed at investigating miR-103a-3p expression, functional roles and underlying mechanism in regulating Alzheimer’s progression.
Methods: RT-qPCR was used to assessed miR-103a-3p and NPAS3 expression in human neuroblastoma cells. Cell transfection of overexpressed or knocked down genes and CCK-8 assay measured cell viability while RT-qPCR was used to detect proliferation and apoptosis in biomarkers, Ki87 and PCNA, caspase-8 and caspase-3, respectively. Furthermore, luciferase assay was used to evaluate the luciferase activity while western blotting analysis was applied to determine protein biomarkers regarding proliferation and apoptosis.
Results: Expression of miR-103a-3p decreased but NPAS3 increased in AD cell lines. Overexpressed miR-103a-3p attenuated cell viability and NPAS3 bound miR-103a-3p to regulate AD progression. The inhibitory effect of miRNA on cell viability in AD was reversed by NPAS3.
Conclusion: miR-103a-3p/NPAS3 might help to enrich knowledge on treatment of AD.
Keywords: Alzheimer’s development, cell growth, cell proliferation
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