Article Sidebar
Published:
May 14, 2021Keywords:
Article Details
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.
Main Article Content
Antidiabetic effect of hydro-methanol extract of <i>Prunus cerasus</i> L fruits and identification of its bioactive compounds
Abstract
Purpose: To investigate the antidiabetic effect of hydro-methanol extract of Prunus cerasus fruit extract.
Methods: The antidiabetic activity was assessed in alloxan-induced diabetic rats. The effect of P. cerasus fruit extract on plasma fasting blood glucose (FBG), insulin, C-peptide, total protein, glycosylated hemoglobin (HbA1c), total hemoglobin, reduced glutathione (GSH), vitamins E and C, ceruloplasmin, lipid profile, histology of the pancreas, and expression of glucose transporter type 4 (GLUT-4) were determined using standard procedures. Liquid chromatography-mass spectrometry was used for phytochemical analysis.
Results: Alloxan-induced diabetes significantly reduced plasma levels of insulin, C-peptide, total hemoglobin and total protein, and significantly increased FBG and HbA1c levels (p < 0.05). However, after treatment with the extract, changes in the levels of these parameters were significantly and dosedependently reversed (p < 0.05). The extract also increased the levels of GSH, vitamins E and C, and. Alloxan-induced DM significantly increased the levels of triacylglycerols (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), and significantly reduced the level of total cholesterol (TC) at different time points (p < 0.05). However, after treatment, the levels of TG, LDL-C and HDL-C declined but TC level was significantly elevated time- and dose-dependently by the extract (p< 0.05). The extract upregulated the expression of GLUT-4 mRNA in soleus muscle and adipose tissue. LC-MS analysis revealed that the extract contained chlorogenic acid, rutin, diadzin, amygdalin, quercetin and naringenin.
Conclusion: The results obtained in this study have shown that hydromethanol extract of P. cerasus fruits exhibits remarkable antidiabetic effects.
