Aqueous Allium sativum (garlic) extract ameliorated CdCl2- induced alterations in blood formation and spermatogenesis in albino rats

  • Edmund Chidiebere Mbegbu
  • Rita Ifeoma Odo
  • Paul Tobechukwu Ozioko
  • Mark Ebubechukwu Awachie
  • Lotanna Gilbert Nwobi
  • Ikechukwu Reginald Obidike
Keywords: Allium sativum, CdCl2 toxicity, Haematology, Spermatogenesis, Rats

Abstract

Purpose: To investigate the ameliorative effect of aqueous garlic extract (AGEx) on cadmium chloride (CdCl2-induced) alterations in the blood and testicles of rats.

Methods: A total of 24 male rats (160 - 200 g), randomly assigned into 4 groups (A - D; n = 6), were used to investigate the claimed protective effect of AGEx on blood and spermatogenic tissues following CdCl2-intoxication in albino rats. The rats in Group A served as controls and were given 5 mg/mL of deionized water. Group B rats were given 300 mg/kg of AGEx. Group C rats were given 2 mg/kg of CdCl2. Rats in Group D first received 2 mg/kg of CdCl2, and 300 mg/kg of AGEx 2 h later. All treatments were done every 48 h for a period of six weeks.

Results: CdCl2 administration to group C rats reduced (p < 0.05) haematocrit value (PCV), concentration of haemoglobin (Hb), red cells count (RBC), total leucocytes count (tWBC), eosinophil, neutrophil, testicular weights and sperm reserves; but elevated (p < 0.05) lymphocytes count compared with the controls. AGEx 300 mg/kg in group D rats significantly reversed (p < 0.05) the altered parameters compared with the controls.

Conclusion: The results demonstrate that administration of aqueous Allium sativum (garlic) extract to male rats enhances spermatogenesis, and ameliorates testicular and haematological alterations induced by cadmium poisoning. Therefore, the spermatogenic principle in AGEx is a potential candidate for the clinical management of male infertility.

Published
2022-01-13
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996