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Published:
Feb 7, 2022Keywords:
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Main Article Content
Metabolic determination of decursinol using human liver microsome
Abstract
Purpose: To determine new metabolites of the main components of Angelica gigas known to give anti-inflammation and pain relief
Methods: Decursinol and blank sample were metabolized in human liver microsomes. The metabolized samples were centrifuged and deproteinated by adding 3 mL acetonitrile. The acetonitrile layer was concentrated and reconstituted in methanol. Finally, the prepared sample was injected into the LC-Q- TOF-MS.
Results: Four new metabolites of decursinol with m/z ranging from 263.0912 ~ 263.0920 were identified as hydroxylated forms of decursinol, and the hydroxylated position of each metabolite was characterized using TOF mass spectrum. Their error values of detected m/z were 0.38 ~ 2.29 ppm, which indicates high accuracy of analysis.
Conclusion: Previously unreported decursinol metabolites have been identified in this study. The findings provide athe basis for further pharmaceutical studies and functional food development using decursinol.
