Achillea millefolium Aqueous Extract does not Impair Recognition Memory in Mice
Purpose: To investigate the effect of the aqueous extract of Achillea millefolium on recognition memory in mice.
Methods: Male mice (35) were used. The aqueous extract of A. millefolium was prepared using a Soxhlet apparatus and injected intraperitoneally in a dose of 50, 250, 500 or 1000 mg/kg daily for 20 days. The control group was treated with saline 1 ml/mouse/day. Tactile learning was assessed using the novel object recognition test (NORT) in a dark room which entailed measurement of the distance travelled during trial and test phases.
Results: Treatment with different doses of A. millefolium did not affect activity levels (based on the distance travelled during trial and test phases). The total time and frequency of visits to the sample objects in trial and test phases were not statistically significant between control and A. millefolium treated groups (p > 0.05). A comparison of the discrimination ratio between the experimental groups revealed no difference. Administration of A. millefolium extract for 20 days did not decrease body weight or cause death in the treated animals.
Conclusion: The result of this study demonstrates that chronic treatment with different doses of the aqueous extract of A. millefolium did not impair recognition memory in mice.
Keywords: Recognition, Memory, Learning, Tactile, Achillea millefolium
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.