Optimized Production of Lignin Peroxidase, Manganese Peroxidase and Laccase in Submerged Cultures of Trametes Trogii Using Various Growth Media Compositions

  • F Patrick
  • G Mtui
  • AM Mshandete
  • A Kivaisi
Keywords: Optimization, Trametes trogii, lignin peroxidase, manganese peroxidase, laccase, fermentation, submerged

Abstract

A white-rot fungus, Trametes trogii, was isolated from coastal Tanzania and screened for crude lignolytic enzymes production using Rhemazol Brilliant blue R (RBBR) dye, 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonate (ABTS) and guaiacol in a semi-solid medium. Lignin peroxidase (LiP), manganese peroxidase (MnP) and Laccase (Lac) were detected by pyrogallol and !- napthol solutions, respectively on the guaiacol supplemented solid media. The effect of temperature, pH, carbon, nitrogen, Cu2+, 2,5-xylidine, ferulic acid, varatryl alcohol and Mn2+ in submerged culture fermentations were investigated for maximum enzymes production. After 7 days of incubation, 72-100% oxidation of RBBR, ABTS and guaiacol was observed. With optimized culture conditions, the fungal filtrate had maximum LiP, MnP and Lac activities of 0.18, 4.44 and 593 U/ml, respectively compared to 0.0011, 0.0054 and 2.3 U/ml obtained with non-optimized ones, amounting to 16,264%, 82,122% and 25,683% increase in LiP, MnP and Lac activities, respectively. The enhanced crude enzymes activities, RBBR decolorization and ABTS guaiacol oxidation capabilities of T. trogii show its potential as a source of industrial enzymes for biotechnological applications.

Keywords: Optimization, Trametes trogii, lignin peroxidase, manganese peroxidase, laccase, fermentation, submerged

Tanz. J. Sci. Vol 36, 2010, 1-18

Author Biographies

F Patrick
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania
G Mtui
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania
AM Mshandete
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania
A Kivaisi
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania
Section
Articles

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eISSN: 2507-7961
print ISSN: 0856-1761