The Use of Competitive Enzyme Linked Immuno-Sorbent Assay in Combination with Abattoir Survey for CBPP Surveillance in Nigeria
AbstractA total of 500 lung tissues and sera samples of cattle from CBPP endemic areas was used in this study to determine the effectiveness of combining abattoir survey with competitive Enzyme Linked Immunosorbent Assay (cELISA) as an alternative to Complement Fixation Test (CFT) in providing a better and more reliable information on CBPP surveillance in Nigeria. A total of 94 (18.8%) of the lung tissues examined had lesions (at various stages) suggestive of CBPP, while 137 (27.4%) and 160 (32%) were sero-positive for CFT and cELISA respectively. One hundred and twelve (22.4%) of the cattle were seropositive for both CFT and cELISA, while 25(5.1%) and 48(9.6%) were seropositive for only CFT and cELISA respectively. Five cattle that had lesions suggestive of CBPP were negative by both CFT and cELISA. Meanwhile, no age predisposition was particularly significant to the development of CBPP lesion in this investigation. It is concluded that the use of cELISA along with abattoir survey in the field by training veterinary officers could provide better and more reliable information on the prevalence of CBPP in Nigeria.
Keyword: Contagious bovine pleuropneumonia, abattoir survey, competitive enzyme-linked immunosorbent assay, compliment fixation test, surveillance, Nigeria.
Trop. Vet. Vol. 21 (2) 35-41 (2003)