Possible mechanisms for the anti-ulcer effects of the ethanolic extract of Enantia chlorantha in rats

  • IM Siminialayi Department of Pharmacology, College of Health Sciences, University of Port Harcourt, PMB 5323, Port Harcourt, Nigeria
  • EO Agbaje Department of Pharmacology, College of Medicine, University of Lagos P.M.B. 12003, Lagos, Nigeria
Keywords: <i>Enantia chlorantha</i>, antiulcer, mechanism, acid secretion, cytoprotection

Abstract

An alkaloid derived from Enantia chloranthahas been reported to have antiulcer properties and our unpublished data demonstrate the antiulcer properties of the crude ethanolic extract of E. chlorantha. This study is aimed at identifying a possible mechanism(s) for the antiulcer action of the crude ethanolic extract of E. chlorantha. Indomethacin- and ethanol-induced gastric ulcers were treated prophylactically by administering 300mg/kg of the crude ethanolic extract of E. chlorantha, intragastrically through an inflexible oral cannula. The response obtained was then compared with the effect of 200mg/kg of misoprostol- and 50mg/kg of ranitidine-pretreatment, both administered in the same manner but separately to the experimental animals. The effect of the extract in organ bath concentrations ranging from 0.1 to 6.4mg/ml was also tested on several segments of isolated rabbit ileum tissue. It was found that the extract completely protected against the ulcerogenic effects of absolute ethanol and was more efficacious in this regard, than misoprostol, which conferred 69.95% protection. It however, had about the same protective ability as misoprostol against indomethacin-induced ulcers (Ulcer indices of 30.21% and 31.75%, respectively). In addition, it inhibited the spontaneous contractions of isolated rabbit ileum smooth muscle in a dose-dependent manner. The extract appears to act in a manner very similar to misoprostol (inhibition of acid secretion and enhanced cytoprotection by stimulation of mucus secretion), in addition to inhibiting the spontaneous contractions of rabbit ileal smooth muscle by an as yet unclear mechanism.

Keywords: Enantia chlorantha, antiulcer, mechanism, acid secretion, cytoprotection

Résumé

Un dérivé alkaloid d\' Enantia chloranthaa été reporté avoir des propriété anti-ulcéreux. Et nos données non encore publiées montre cette propriété anti-ulcéreux d\'extrait brute d\' Enantia chloranthacette étude a pour but d\' indendentifier les possible mecanismes d\'action anti-ulcéreux de l\'extrait ethanolique brute d\' Enantia chlorantha. L\'ulcer induite par l\'indomethacine et l\'ethanol ont été traitées prophylactiquement en administrant 300mg/kg d\'extrait ethanolique brute d\' Enantia chlorantha intragstriquement en utilisant des cannules oral non flexible. Les réponses obtenues ont été en suite comparées avec l\'effet du pré-traitement de 200mg/kg de Misoprostol et 50mg/kg de Ranitidine. Tous administrés de la même manière mais séparément aux animaux experimenté l\'effet de L\'extrait sur des bains d\'organe à concentration allant de 0.1 a 6.4mg/ml ont été aussi testé sur different segment de tissue d\'ilieum isolés de lapin. Il en est resulté que l\'extrait protégait completement contre les effet ulcerogénique d\'ethanol absolute et était plus efficace à ce regards que le misoprostol, qui conferait une protection de 69.95%. Ce pendent il eu la même abilité protective que le Misoprostol contre l\'ulcer induite par l\'indomethacine.(l\'indice d\'ulcer de 30.21% et 31.75% respectivement) en plus il inhibait la contraction spontanée des muscles blanc d\'ilieum isolée de lapin et ce à dose dépendent. L\'extrait apparait agir de manière similaire au misoprostol (inhibition de la sécretion d\'acide et augmentation de la cytoprotection par stimulation de la secretion du mucus), en plus l\'inhibait de la contraction spontanée des muscles blanc d\'ilieum isolées de lapin mais par un mécanisme non élucidée.

Mots clés: d\' Enanatia chlorantha, anti-ulcereux, mecanisme, secretion d\'acid, cytoprotection

West African Journal of Pharmacology and Drug Research Vol. 20(1&2) 2005: 39-43

Published
2005-12-05
Section
Articles

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eISSN: 0303-691X